1. This site uses cookies. By continuing to use this site, you are agreeing to our use of cookies. Learn More.
Dismiss Notice
You must be a registered member in order to post messages and view/download attached files in this forum.
Click here to register.

Worst-case protein concentration for degradation study for cleaning

Discussion in 'Manufacturing and Related Processes' started by Sunny, Jan 9, 2018.

  1. Sunny

    Sunny New Member

    Jan 9, 2018
    Likes Received:
    Trophy Points:
    Hi all, I'm new here so this is my very first thread! I'm in the process of setting up a degradation study for a cleaning process and I'm a bit stuck with how to determine the worst-case protein concentration for my bench scale study that represents the actual process. Say I have several chromatography steps and a last filtration step before the product is in it's 'final', then I would take the equipment used for final filtration which I know the protein concentration going in. When cleaning it will have several rinses before the actual cleaning agent, how do I determine the concentration of protein to work with for my bench scale with the cleaning agent? Any suggestions welcome. Thank you!